Affiliation:
1. Department of Microbiology, University of Minnesota, Minneapolis 55455-0312, USA.
Abstract
Ll.ltrB is a functional group II intron located within a gene (ltrB) encoding a conjugative relaxase essential for transfer of the lactococcal element pRSO1. In this work, the Ll.ltrB intron was shown to be an independent mobile element capable of inserting into an intronless allele of the ltrB gene. Ll.ltrB was not observed to insert into a deletion derivative of the ltrB gene in which the intron splice site was removed. In contrast, a second vector containing a 271-nucleotide segment of ltrB spanning the Ll.ltrB splice site was shown to be a proficient recipient of intron insertion. Efficient homing was observed in the absence of a functional host homologous recombination system. This work demonstrates that the Ll.ltrB intron is a novel site-specific mobile element in lactococci and that group II intron self-transfer is a mechanism for intron dissemination among bacteria.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference34 articles.
1. Isolation of a recombinationdeficient mutant of Streptococcus lactis ML3;Anderson D. G.;J. Bacteriol.,1983
2. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl (ed.). 1995. Current protocols in molecular biology. John Wiley & Sons Inc. New York N.Y.
3. Prokaryotic introns and inteins: a panoply of form and function;Belfort M.;J. Bacteriol.,1995
4. Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons;Clewell D. B.;Trends Microbiol.,1995
5. Retrohoming: cDNA-mediated mobility of group II introns requires a catalytic RNA;Curcio M. J.;Cell,1996
Cited by
72 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献