Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase-Reference-Cited by-同舟云学术

Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase

Published:1997-09 Issue:18 Volume:179 Page:5963-5966
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Zheng L¹,White R H¹,Dean D R¹

Affiliation:

1. Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg 24061-0346, USA.

Abstract

The nifV gene product (NifV) from Azotobacter vinelandii was recombinantly expressed at high levels in Escherichia coli and purified. NifV is a homodimer that catalyzes the condensation of acetyl coenzyme A (acetyl-CoA) and alpha-ketoglutarate. Although alpha-ketoglutarate supports the highest level of activity, NifV will also catalyze the condensation of acetyl-CoA and certain other keto acids. E. coli cells in which a high level of nifV expression is induced excrete homocitrate into the growth medium.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.179.18.5963-5966.1997

Reference21 articles.

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