Real-Time PCR Assay for Differentiation of Typhoidal and Nontyphoidal Salmonella

Author:

Nair Satheesh1ORCID,Patel Vineet12,Hickey Tadgh12,Maguire Clare1,Greig David R.1,Lee Winnie1,Godbole Gauri1,Grant Kathie1,Chattaway Marie Anne1

Affiliation:

1. Gastrointestinal Bacteria Reference Unit, Public Health England, London, United Kingdom

2. School of Life and Medical Sciences, University of Hertfordshire, Hatfield, United Kingdom

Abstract

Rapid and accurate differentiation of Salmonella spp. causing enteric fever from nontyphoidal Salmonella is essential for clinical management of cases, laboratory risk management, and implementation of public health measures. Current methods used for confirmation of identification, including biochemistry and serotyping as well as whole-genome sequencing analyses, take several days.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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