Comparison of Traditional Phenotypic Identification Methods with Partial 5′ 16S rRNA Gene Sequencing for Species-Level Identification of Nonfermenting Gram-Negative Bacilli-Reference-Cited by-同舟云学术

Comparison of Traditional Phenotypic Identification Methods with Partial 5′ 16S rRNA Gene Sequencing for Species-Level Identification of Nonfermenting Gram-Negative Bacilli

Published:2010-04 Issue:4 Volume:48 Page:1442-1444
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cloud Joann L.¹,Harmsen Dag²,Iwen Peter C.³,Dunn James J.⁴,Hall Gerri⁵,LaSala Paul Rocco⁶,Hoggan Karen¹,Wilson Deborah⁵,Woods Gail L.¹⁷,Mellmann Alexander⁸

Affiliation:

1. ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah

2. Department of Periodontology, University of Münster, Münster, Germany

3. Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska

4. Cook Children's Medical Center, Fort Worth, Texas

5. Section of Clinical Microbiology, Cleveland Clinic, Cleveland, Ohio

6. Department of Pathology and Clinical Microbiology, University of Texas Medical Branch, Galveston, Texas

7. Department of Pathology, University of Utah, Salt Lake City, Utah

8. Institute of Hygiene, University of Münster, Münster, Germany

Abstract

ABSTRACT Correct identification of nonfermenting Gram-negative bacilli (NFB) is crucial for patient management. We compared phenotypic identifications of 96 clinical NFB isolates with identifications obtained by 5′ 16S rRNA gene sequencing. Sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify NFB.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00169-10

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