Abstract
Polyoma (Py) virus multiplies, at 34 and 38.5 C, in wild-type (WT-4) and in ts A1S9 mouse L cells, which are temperature sensitive for growth and for DNA replication (R. Sheinin, 1976; L. H. Thompson et al., 1970). De novo synthesis of double-stranded, fully covalently closed Py DNA has been shown to proceed by semiconservative replication in WT-4 and ts A1S9 cells at the permissive and nonpermissive temperatures. Cell DNA is made late during infection, by both cell types and at both temperatures. Semiconservative replication of cell DNA proceeds in Py-infected WT-4 cells incubated at 34 or at 38.5 C and in Py-infected ts A1S9 cells incubated at 34 C. In virus-infected ts A1S9 cells incubated at 38.5 C, cell DNA synthesis appears to proceed almost entirely by a process analogous to repair replication. The inability of ts A1S9 cells to produce large-molecular-weight chromosomal DNA strands, at 38.5 C, by the normal mechanism is not overcome by Py infection.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference47 articles.
1. Adams M. H. 1959. Bacteriophages. Interscience New York.
2. Growth of SE polyoma virus in primary and established cell cultures;Balduzzi P.;Proc. Soc. Exp. Biol. Med.,1960
3. Response of different mouse cell strains to polyoma infection in vitro. Latency and self-inhibition effect in infected cultures;Barski G.;J. Natl. Cancer Inst.,1962
4. Heterogeneity of polyoma virus DNA: isolation and characterization of noninfectious small supercoiled molecules;Blackstein M. E.;J. Mol. Biol.,1969
5. Is a specific protein responsible for the supercoiling of polyoma DNA;Bourgaux P.;Nature (London),1972
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