A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus cereus Group Is Diagnostic for Bacillus anthracis-Reference-Cited by-同舟云学术

A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus cereus Group Is Diagnostic for Bacillus anthracis

Published:1999-03 Issue:3 Volume:65 Page:1298-1303
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Daffonchio Daniele¹,Borin Sara¹,Frova Giuseppe¹,Gallo Romina²,Mori Elena²,Fani Renato²,Sorlini Claudia¹

Affiliation:

1. Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, 20133 Milan,1 and

2. Dipartimento di Biologia Animale e Genetica, Università degli Studi, 50125 Florence,2 Italy

Abstract

ABSTRACT Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus , Bacillus thuringiensis , and Bacillus mycoides , we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus , including 61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850) specific for B. cereus , B. thuringiensis , B. anthracis , and B. mycoides was identified. This fragment included a putative (366-nucleotide) open reading frame highly homologous to the ypuA gene of Bacillus subtilis . The restriction analysis of the SG-850 fragment with Alu I distinguished B. anthracis from the other species of the B. cereus group.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.3.1298-1303.1999

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