Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis-Reference-Cited by-同舟云学术

Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis

Published:2002-08 Issue:8 Volume:46 Page:2720-2722
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Palomino Juan-Carlos¹,Martin Anandi¹,Camacho Mirtha²,Guerra Humberto³,Swings Jean⁴⁵,Portaels Françoise¹

Affiliation:

1. Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000

2. Tuberculosis Laboratory, Instituto Nacional de Laboratorios de Salud, La Paz, Bolivia

3. Instituto de Medicina Tropical “Alexander von Humboldt,” Universidad Peruana Cayetano Heredia, Lima 100, Peru

4. Laboratorium voor Microbiologie

5. BCCM/LMG Culture Collection, Universiteit Gent, B-9000 Ghent, Belgium

Abstract

ABSTRACT A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.46.8.2720-2722.2002

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