Characterization of a Serodiagnostic Complement Fixation Antigen of Coccidioides posadasii Expressed in the Nonpathogenic Fungus Uncinocarpus reesii

Abstract

ABSTRACT Coccidioides spp. ( immitis and posadasii ) are the causative agents of human coccidioidomycosis. In this study, we developed a novel system to overexpress coccidioidal proteins in a nonpathogenic fungus, Uncinocarpus reesii , which is closely related to Coccidioides . A promoter derived from the heat shock protein gene ( HSP60 ) of Coccidioides posadasii was used to control the transcription of the inserted gene in the constructed coccidioidal protein expression vector (pCE). The chitinase gene ( CTS1 ) of C. posadasii , which encodes the complement fixation antigen, was expressed using this system. The recombinant Cts1 protein (rCts1 Ur ) was induced in pCE-CTS1-transformed U. reesii by elevating the cultivation temperature. The isolated rCts1 Ur showed chitinolytic activity that was identical to that of the native protein and had serodiagnostic efficacy comparable to those of the commercially available antigens in immunodiffusion-complement fixation tests. Using the purified rCts1 Ur , 74 out of the 77 coccidioidomycosis patients examined (96.1%) were positively identified by enzyme-linked immunosorbent assay. The rCts1 Ur protein showed higher chitinolytic activity and slightly greater seroreactivity than the bacterially expressed recombinant Cts1. These data suggest that this novel expression system is a useful tool to produce coccidioidal antigens for use as diagnostic antigens.