Affiliation:
1. Division of Medicinal and Natural Products Chemistry, and Center for Biocatalysis and Bioprocessing, College of Pharmacy, University of Iowa, Iowa City, Iowa 52242
Abstract
ABSTRACT
The conversions of vanillic acid and
O
-benzylvanillic acid to vanillin were examined by using whole cells and enzyme preparations of
Nocardia
sp. strain NRRL 5646. With growing cultures, vanillic acid was decarboxylated (69% yield) to guaiacol and reduced (11% yield) to vanillyl alcohol. In resting
Nocardia
cells in buffer, 4-
O
-benzylvanillic acid was converted to the corresponding alcohol product without decarboxylation. Purified
Nocardia
carboxylic acid reductase, an ATP and NADPH-dependent enzyme, quantitatively reduced vanillic acid to vanillin. Structures of metabolites were established by
1
H nuclear magnetic resonance and mass spectral analyses.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
77 articles.
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