Comparison of the BioFire Joint Infection Panel to 16S Ribosomal RNA Gene-Based Targeted Metagenomic Sequencing for Testing Synovial Fluid from Patients with Knee Arthroplasty Failure

Author:

Azad Marisa A.12ORCID,Wolf Matthew J.2,Strasburg Angela P.2,Daniels Matthew L.2,Starkey Jordan C.2,Donadio Alexander D.2,Abdel Matthew P.3,Greenwood-Quaintance Kerryl E.2,Patel Robin12ORCID

Affiliation:

1. Division of Public Health, Infectious Diseases, and Occupational Medicine, Department of Medicine, Mayo Clinic, Rochester, Minnesota, USA

2. Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA

3. Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA

Abstract

The diagnosis of periprosthetic joint infection (PJI) is challenging, often requiring multiple clinical specimens and diagnostic techniques, some with prolonged result turnaround times. Here, the diagnostic performance of the Investigational Use Only (IUO) BioFire Joint Infection (JI) Panel was compared to 16S rRNA gene-based targeted metagenomic sequencing (tMGS) applied to synovial fluid for PJI diagnosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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