Detection of Viruses in Clinical Samples by Use of Metagenomic Sequencing and Targeted Sequence Capture-Reference-Cited by-同舟云学术

Detection of Viruses in Clinical Samples by Use of Metagenomic Sequencing and Targeted Sequence Capture

Published:2018-12 Issue:12 Volume:56 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Wylie Kristine M.¹²^ORCID,Wylie Todd N.¹²,Buller Richard¹,Herter Brandi¹,Cannella Maria T.¹,Storch Gregory A.¹

Affiliation:

1. Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri, USA

2. The McDonnell Genome Institute, Washington University School of Medicine, St. Louis, Missouri, USA

Abstract

Metagenomic shotgun sequencing (MSS) is a revolutionary approach to viral diagnostic testing that allows simultaneous detection of a broad range of viruses, detailed taxonomic assignment, and detection of mutations associated with antiviral drug resistance. To enhance sensitivity for virus detection, we previously developed ViroCap, a targeted sequence capture panel designed to enrich nucleic acid from a comprehensive set of eukaryotic viruses prior to sequencing.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01123-18

Reference52 articles.

1. Unbiased Detection of Respiratory Viruses by Use of RNA Sequencing-Based Metagenomics: a Systematic Comparison to a Commercial PCR Panel

2. Actionable Diagnosis of Neuroleptospirosis by Next-Generation Sequencing

3. Direct multiplexed whole genome sequencing of respiratory tract samples reveals full viral genomic information