Involvement of an Essential Gene, mviN , in Murein Synthesis in Escherichia coli

Author:

Inoue Azusa1,Murata Yoshimitsu2,Takahashi Hiroshi2,Tsuji Naoko1,Fujisaki Shingo2,Kato Jun-ichi1

Affiliation:

1. Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan

2. Department of Biomolecular Science, Faculty of Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan

Abstract

ABSTRACT We isolated a temperature-sensitive mutant with a mutation in mviN , an essential gene in Escherichia coli . At the nonpermissive temperature, mviN mutant cells swelled and burst. An intermediate in murein synthesis, polyprenyl diphosphate- N -acetylmuramic acid-(pentapeptide)- N -acetyl-glucosamine, accumulated in mutant cells. These results indicated that MviN is involved in murein synthesis.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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