Affiliation:
1. Syngenta Biotechnology, Inc., 3054 Cornwallis Rd., Research Triangle Park, NC 27709
2. Syngenta Crop Protection AG, Schwarzwaldallee 215, CH-4002 Basel, Switzerland
Abstract
ABSTRACT
The cytochrome P450 monooxygenase Ema1 from
Streptomyces tubercidicus
R-922 and its homologs from closely related
Streptomyces
strains are able to catalyze the regioselective oxidation of avermectin into 4"-oxo-avermectin, a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate (V. Jungmann, I. Molnár, P. E. Hammer, D. S. Hill, R. Zirkle, T. G. Buckel, D. Buckel, J. M. Ligon, and J. P. Pachlatko, Appl. Environ. Microbiol.
71:
6968-6976, 2005). The gene for Ema1 has been expressed in
Streptomyces lividans
,
Streptomyces avermitilis
, and solvent-tolerant
Pseudomonas putida
strains using different promoters and vectors to provide biocatalytically competent cells. Replacing the extremely rare TTA codon with the more frequent CTG codon to encode Leu4 in Ema1 increased the biocatalytic activities of
S. lividans
strains producing this enzyme. Ferredoxins and ferredoxin reductases were also cloned from
Streptomyces coelicolor
and biocatalytic
Streptomyces
strains and tested in
ema1
coexpression systems to optimize the electron transport towards Ema1.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
20 articles.
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