Affiliation:
1. Department of Oceanography, Florida State University, Tallahassee, Florida
2. Marine Microbiology Laboratory, Korea Ocean Research and Development Institute, Seoul, Korea
Abstract
ABSTRACT
To elucidate the geomicrobiological factors controlling nitrification in salt marsh sediments, a comprehensive approach involving sediment geochemistry, process rate measurements, and quantification of the genetic potential for nitrification was applied to three contrasting salt marsh habitats: areas colonized by the tall (TS) or short (SS) form of
Spartina alterniflora
and unvegetated creek banks (CBs). Nitrification and denitrification potential rates were strongly correlated with one another and with macrofaunal burrow abundance, indicating that coupled nitrification-denitrification was enhanced by macrofaunal burrowing activity. Ammonia monooxygenase (
amoA
) gene copy numbers were used to estimate the ammonia-oxidizing bacterial population size (5.6 × 10
4
to 1.3 × 10
6
g of wet sediment
−1
), which correlated with nitrification potentials and was 1 order of magnitude higher for TS and CB than for SS. TS and CB sediments also had higher Fe(III) content, higher Fe(III)-to-total reduced sulfur ratios, higher Fe(III) reduction rates, and lower dissolved sulfides than SS sediments. Iron(III) content and reduction rates were positively correlated with nitrification and denitrification potential and
amoA
gene copy number. Laboratory slurry incubations supported field data, confirming that increased amounts of Fe(III) relieved sulfide inhibition of nitrification. We propose that macrofaunal burrowing and high concentrations of Fe(III) stimulate nitrifying bacterial populations, and thus may increase nitrogen removal through coupled nitrification-denitrification in salt marsh sediments.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference51 articles.
1. Aller, R. C. 1988. Benthic fauna and biogeochemical processes in marine sediments: the role of burrow structures, p. 301-338. In T. H. Blackburn and J. Sorensen (ed.), Nitrogen cycling in coastal marine environments, vol. 33. John Wiley & Sons, New York, N.Y.
2. Alongi D. M. 1998. Coastal ecosystem processes p. 43-92. CRC Press Boca Raton Fla.
3. Anderson, I. C., C. R. Tobias, B. B. Neikirk, and R. L. Wetzel. 1997. Development of a process-based nitrogen mass balance model for a Virginia (USA) Spartina alterniflora salt marsh: implications for net DIN flux. Mar. Ecol. Prog. Ser.159:13-27.
4. Specific Inhibition of Nitrite Oxidation by Chlorate and Its Use in Assessing Nitrification in Soils and Sediments
5. Bjerrum, L., T. Kjaer, and N. B. Ramsing. 2002. Enumerating ammonia-oxidizing bacteria in environmental samples using competitive PCR. J. Microbiol. Methods51:227-239.
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