Legionella Confirmation Using Real-Time PCR and SYTO9 Is an Alternative to Current Methodology-Reference-Cited by-同舟云学术

Legionella Confirmation Using Real-Time PCR and SYTO9 Is an Alternative to Current Methodology

Published:2005-12 Issue:12 Volume:71 Page:8944-8948
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Giglio Steven¹,Monis Paul T.¹,Saint Christopher P.¹

Affiliation:

1. Australian Water Quality Centre, South Australian Water Corporation, Salisbury, South Australia 5108, Australia

Abstract

ABSTRACT The currently accepted culture techniques for the detection of Legionella spp. in water samples (AS/NZS 3896:1998 and ISO 11731 standard methods) are slow and laborious, requiring from 7 to 14 days for a result. We describe a fully validated rapid confirmation technique that uses real-time PCR incorporating the intercalating dye SYTO9 for the direct identification of primary cultures, significantly decreasing turnaround time and allowing faster remedial action to be taken by the industry.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.12.8944-8948.2005

Reference19 articles.

1. Performance-based maintenance of cooling water systems 2000

2. Anonymous. 1998. Water quality—detection and enumeration of Legionella. ISO 11731:1998. International Organisation for Standardisation Geneva Switzerland.

3. Anonymous. 1998. Waters—examination for Legionellae. AS/NZS 3896:1998. Standards Australia North Sydney NSW Australia.

4. Atlas, R. M. 1999. Legionella: from environmental habitats to disease pathology, detection and control. Environ Microbiol.1:283-293.

5. Bartie, C., S. N. Venter, and L. H. Nel. 2003. Identification methods for Legionella from environmental samples. Water Res.37:1362-1370.

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