Scarce Evidence of Yogurt Lactic Acid Bacteria in Human Feces after Daily Yogurt Consumption by Healthy Volunteers

Author:

del Campo Rosa1,Bravo Daniel1,Cantón Rafael1,Ruiz-Garbajosa Patricia1,García-Albiach Raimundo2,Montesi-Libois Alejandra2,Yuste Francisco-Javier1,Abraira Victor1,Baquero Fernando1

Affiliation:

1. Departments of Microbiology, Preventive Medicine, and Biostatistics, Ramón y Cajal University Hospital

2. Department of Microbiology, San Pablo-CEU University, Madrid, Spain

Abstract

ABSTRACT In a double-blind prospective study including 114 healthy young volunteers, the presence in human feces of the yogurt organisms Lactobacillus delbrueckii and Streptococcus thermophilus after repeated yogurt consumption (15 days) was analyzed by culture, specific PCR, and DNA hybridization of total fecal DNA. Detection of yogurt lactic acid bacteria in total fecal DNA by bacterial culture and PCR assay was consistently negative. DNA compatible with yogurt bacteria was found by hybridization experiments in only 10 (10.52%) of 96 individuals after consumption of fresh yogurt and in 2 (2.10%) of 96 individuals after consumption of pasteurized yogurt ( P = 0.01).

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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5. Fleiss J. L. 1986. The design and analysis of clinical experiments. John Wiley & Sons Inc. New York N.Y.

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