Detection of Francisella tularensis in blood by polymerase chain reaction-Reference-Cited by-同舟云学术

Detection of Francisella tularensis in blood by polymerase chain reaction

Published:1993-01 Issue:1 Volume:31 Page:152-154
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Long G W¹,Oprandy J J¹,Narayanan R B¹,Fortier A H¹,Porter K R¹,Nacy C A¹

Affiliation:

1. Center for Immunization Research, Johns Hopkins University, Baltimore, Maryland 21205.

Abstract

We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.31.1.152-154.1993

Reference15 articles.

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