Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids-Reference-Cited by-同舟云学术

Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids

Published:1989-04 Issue:4 Volume:171 Page:2241-2243
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Flinn H¹,Burke M¹,Stirling C J¹,Sherratt D J¹

Affiliation:

1. Institute of Genetics, University of Glasgow, United Kingdom.

Abstract

Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1 site-specific recombination.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.171.4.2241-2243.1989

Reference17 articles.

1. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in E. coli;Amann C.;Gene,1983

2. Polar mobilization of the Escherichia coli chromosome by the ColEl transfer origin;Boyd A. C.;Mol. Gen. Genet.,1986

3. Trimethoprim resistance conferred by IncW plasmids in Enterobacteriaciae;Datta N.;J. Gen. Microbiol.,1972

4. Replacement and amplification of bacterial genes with sequences altered in vitro;Gutterson N. I.;Proc. Natl. Acad. Sci. USA,1983

5. Hakkaart M. J. J. P. J. M. Van den Elzen E. Veltkamp and H. J. J. Nikjamp. 1984. Maintenance of multicopy plasmid CloDF13 in E. coli cells: evidence for site-specific recombina-NOTES 2243

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