Affiliation:
1. State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China
2. School of Chemical Engineering & Environment, Beijing Institute of Technology, Beijing, China
3. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
Abstract
ABSTRACT
Stenotrophomonas maltophilia
is a Gram-negative bacterial pathogen of increasing concern to human health. Most clinical isolates of
S. maltophilia
efficiently form biofilms on biotic and abiotic surfaces, making this bacterium resistant to a number of antibiotic treatments and therefore difficult to eliminate. To date, very few studies have investigated the molecular and regulatory mechanisms responsible for
S. maltophilia
biofilm formation. Here we constructed a random transposon insertion mutant library of
S. maltophilia
ATCC 13637 and screened 14,028 clones. A total of 46 nonredundant genes were identified. Mutants of these genes exhibited marked changes in biofilm formation, suggesting that multiple physiological pathways, including extracellular polysaccharide production, purine synthesis, transportation, and peptide and lipid synthesis, are involved in bacterial cell aggregation. Of these genes, 20 putatively contributed to flagellar biosynthesis, indicating a critical role for cell motility in
S. maltophilia
biofilm formation. Genetic and biochemical evidence demonstrated that an orphan response regulator, FsnR, activated transcription of at least two flagellum-associated operons by directly binding to their promoters. This regulatory protein plays a fundamental role in controlling flagellar assembly, cell motility, and biofilm formation. These results provide a genetic basis to systematically study biofilm formation of
S. maltophilia
.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
41 articles.
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