Modified Multiplex PCR Method for Detection of Pyrogenic Exotoxin Genes in Staphylococcal Isolates

Author:

Løvseth Astrid1,Loncarevic Semir1,Berdal Knut G.1

Affiliation:

1. Section of Feed and Food Microbiology, National Veterinary Institute, Ullevålsveien 68, 0033 Oslo, Norway

Abstract

ABSTRACT A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes ( sea , seb , sec , sed , see , seg , seh , sei , and sej ) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37: 3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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