Affiliation:
1. Infectious Disease Research, Abbott Laboratories, Abbott Park, Illinois
Abstract
ABSTRACT
Recent work has shown that the efflux genes in
Streptococcus pneumoniae
that are responsible for acquired macrolide resistance can be distinguished as either
mef
(E) or
mef
(A). The genetic elements on which
mef
(A) and
mef
(E) are found also carry an open reading frame (ORF) that is 56% homologous to
msr
(A) in
Staphylococcus
. The prevalence of
mef
(A/E) and of the
msr
-like ORF [
msr
(D)] was evaluated in 153
mef
+
S. pneumoniae
clinical isolates collected in North America, Europe, Africa, and Asia from 1997 to 2002. Clinical isolates were screened with PCR primers specific for either
mef
(A) or
mef
(E) and for
msr
(D).
mef
(A),
mef
(E), and
msr
(D) were cloned from
mef
+
strains and transformed into a susceptible, competent strain of
S. pneumoniae
. The transformants were tested for antimicrobial susceptibilities and efflux pump induction. The results of this work demonstrated that
mef
(A) is more often isolated in parts of Europe, with some incidence in Canada, and that the
msr
-like gene alone can confer the efflux phenotype.
Publisher
American Society for Microbiology
Cited by
96 articles.
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