Selected Pool of Peptides from ESAT-6 and CFP-10 Proteins for Detection of Mycobacterium tuberculosis Infection-Reference-Cited by-同舟云学术

Selected Pool of Peptides from ESAT-6 and CFP-10 Proteins for Detection of Mycobacterium tuberculosis Infection

Published:2004-08 Issue:8 Volume:42 Page:3469-3474
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Scarpellini Paolo¹,Tasca Silvana¹²,Galli Laura³,Beretta Alberto¹,Lazzarin Adriano¹⁴,Fortis Claudio¹²

Affiliation:

1. Clinic of Infectious Diseases

2. Laboratory of Clinical Immunology

3. San Raffaele Hospital “Vita-Salute” San Raffaele University

4. San Raffaele Scientific Institute, Milan, Italy

Abstract

ABSTRACT We have validated a new test for detecting Mycobacterium tuberculosis infection. A pool of synthetic peptides derived from ESAT-6 and CFP-10 proteins was used to detect the number of specific gamma interferon-producing T cells by means of an enzyme-linked immunospot assay. Sixty-eight individuals positive for M. tuberculosis infection, either human immunodeficiency virus-seropositive or -seronegative, were studied. The test results were highly specific (87.5%) and sensitive (93.1%), more so than a classical lymphoproliferative assay (specificity and sensitivity of 77.27%), opening new possibilities for diagnosis and screening of tuberculosis. Moreover, the test allowed us to distinguish individuals infected with M. tuberculosis from those vaccinated with BCG.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.8.3469-3474.2004

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