Use of 16S rRNA Gene Sequencing for Rapid Confirmatory Identification of Brucella Isolates-Reference-Cited by-同舟云学术

Use of 16S rRNA Gene Sequencing for Rapid Confirmatory Identification of Brucella Isolates

Published:2004-08 Issue:8 Volume:42 Page:3649-3654
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Gee Jay E.¹,De Barun K.¹,Levett Paul N.¹,Whitney Anne M.¹,Novak Ryan T.¹,Popovic Tanja¹

Affiliation:

1. Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Abstract

ABSTRACT Members of the genus Brucella are categorized as biothreat agents and pose a hazard for both humans and animals. Current identification methods rely on biochemical tests that may require up to 7 days for results. We sequenced the 16S rRNA genes of 65 Brucella strains along with 17 related strains likely to present a differential diagnostic challenge. All Brucella 16S rRNA gene sequences were determined to be identical and were clearly different from the 17 related strains, suggesting that 16S rRNA gene sequencing is a reliable tool for rapid genus-level identification of Brucella spp. and their differentiation from closely related organisms.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.8.3649-3654.2004

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