Gene Dosage for 5 S Ribosomal Ribonucleic Acid in Escherichia coli and Bacillus megaterium-Reference-Cited by-同舟云学术

Gene Dosage for 5 S Ribosomal Ribonucleic Acid in Escherichia coli and Bacillus megaterium

Published:1971-01 Issue:1 Volume:105 Page:142-149
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Pace B.¹,Pace N. R.¹

Affiliation:

1. Division of Research, National Jewish Hospital and Research Center, and Department of Biophysics, University of Colorado School of Medicine, Denver, Colorado 80206

Abstract

The number of gene copies for 5 S ribosomal ribonucleic acid (rRNA), relative to that for 16 and 23 S rRNA, has been determined by deoxyribonucleic acid (DNA)-RNA hybridization for Escherichia coli and Bacillus megaterium . In both cases, the number of 5 S rRNA genes equals the number of 16 or 23 S rRNA genes. Rapid procedures for preparing extremely highly purified DNA suitable for DNA-RNA hybridization experiments and chemically pure 5 S rRNA are described.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.105.1.142-149.1971

Reference17 articles.

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