An RNA Hairpin Sequesters the Ribosome Binding Site of the Homing Endonuclease mobE Gene-Reference-Cited by-同舟云学术

An RNA Hairpin Sequesters the Ribosome Binding Site of the Homing Endonuclease mobE Gene

Published:2009-04 Issue:7 Volume:191 Page:2409-2413
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Gibb Ewan A.¹,Edgell David R.¹

Affiliation:

1. Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, Ontario, Canada N6A 5C1

Abstract

ABSTRACT Previous transcript mapping of the bacteriophage Aeh1 nrd operon revealed a predicted RNA hairpin upstream of the homing endonuclease mobE gene. We enzymatically mapped the hairpin, showing that the mobE ribosome binding site is sequestered. Cloning of the hairpin upstream of lacZ resulted in reduced β-galactosidase activity, consistent with translational regulation.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.01751-08

Reference29 articles.

1. Barth, K. A., D. Powell, M. Trupin, and G. Mosig. 1988. Regulation of two nested proteins from gene 49 (recombination endonuclease VII) and of a lambda RexA-like protein of bacteriophage T4. Genetics 120 : 329-343.

2. Belfort, M., V. Derbyshire, B. Cousineau, and A. Lambowitz. 2002. Mobile introns: pathways and proteins, p. 761-783. In N. Craig, R. Craigie, M. Gellert, and A. Lambowitz (ed.), Mobile DNA II. ASM Press, Washington, DC.

3. Belle, A., M. Landthaler, and D. A. Shub. 2002. Intronless homing: site-specific endonuclease SegF of bacteriophage T4 mediates localized marker exclusion analogous to homing endonucleases of group I introns. Genes Dev. 16 : 351-362.

4. Brok-Volchanskaya, V. S., F. A. Kadyrov, D. E. Sivogrivov, P. M. Kolosov, A. S. Sokolov, M. G. Shlyapnikov, V. M. Kryukov, and I. E. Granovsky. 2008. Phage T4 SegB protein is a homing endonuclease required for the preferred inheritance of T4 tRNA gene region occurring in co-infection with a related phage. Nucleic Acids Res. 36 : 2094-2105.

5. Isolation and Partial Characterization of Two Aeromonas hydrophila Bacteriophages

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