Directed Mutagenesis and Plasmid-Based Complementation in the Methanogenic Archaeon Methanosarcina acetivorans C2A Demonstrated by Genetic Analysis of Proline Biosynthesis-Reference-Cited by-同舟云学术

Directed Mutagenesis and Plasmid-Based Complementation in the Methanogenic Archaeon Methanosarcina acetivorans C2A Demonstrated by Genetic Analysis of Proline Biosynthesis

Published:2002-03 Issue:5 Volume:184 Page:1449-1454
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Zhang Jun Kai¹,White Andrea K.¹,Kuettner H. Craig¹,Boccazzi Paolo¹,Metcalf William W.¹

Affiliation:

1. Department of Microbiology, University of Illinois, Urbana, Illinois 61801

Abstract

ABSTRACT We report here the first use of directed mutagenesis in Methanosarcina acetivorans C2A. The method employs homologous recombination-mediated gene replacement and was used to construct a variety of proline auxotrophs with mutations in the proABC locus. Each mutation was also complemented in trans with autonomously replicating Methanosarcina - Escherichia plasmid shuttle vectors.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.184.5.1449-1454.2002

Reference24 articles.

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