Affiliation:
1. Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Federal Republic of Germany.
Abstract
The genome of hepatitis A virus (HAV) was reverse transcribed into cDNA and molecularly cloned. cDNA clones coding for the capsid protein VP1 that carries the major HAV antigen were cloned into the expression vector pUR290 and expressed in Escherichia coli. The recombinant fusion protein reacted in an immunoblot with rabbit anti-HAV serum, suggesting that it possesses HAV antigenicity.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference19 articles.
1. Sequence analysis of hepatitis A virus cDNA coding for capsid proteins and RNA polymerase;Baroudy B. M.;Proc. Natl. Acad. Sci. USA,1985
2. Evidence that the genome of hepatitis A virus consists of a single-stranded RNA;Coulepis A. G.;J. Virol.,1981
3. Induction of hepatitis A virus-neutralizing antibody by a virusspecific synthetic peptide;Emini E. A.;J. Virol.,1985
4. Propagation of human hepatitis A virus in a hepatoma cell line;Frosner G. G.;Infection,1979
5. Gauss-Muller V. and F. Deinhardt. 1985. Immunochemical studies on hepatitis A virus p. 273-282. In K. Nishioka B. Blumenberg N. Ishida K. Koike (ed.) Hepatitis viruses and hepatocellular carcinoma. Academic Press Inc. New York.
Cited by
28 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献