Effect of DNase and Antibiotics on Biofilm Characteristics-Reference-Cited by-同舟云学术

Effect of DNase and Antibiotics on Biofilm Characteristics

Published:2009-03 Issue:3 Volume:53 Page:1204-1209
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Tetz George V.¹,Artemenko Natalia K.¹,Tetz Victor V.¹

Affiliation:

1. Department of Microbiology, Virology, and Immunology, St. Petersburg State Pavlov Medical University, 6/8, Tolstoy Str., St. Petersburg 197089, Russia

Abstract

ABSTRACT The role of extracellular DNA in the maintenance of biofilms formed by gram-positive and gram-negative bacteria was studied. This study evaluated all the bacterial strains that were tested for the presence of extracellular DNA with an average size of 30 kb in the matrix. Our results indicate changes in community biomass, architecture, morphology, and the numbers of CFU in the presence of DNase. This effect seems to be common to biofilms established by various unrelated gram-positive and gram-negative bacteria. The cleavage of extracellular DNA leads to the formation of an altered biofilm that permits the increased penetration of antibiotics. Thus, the addition of DNase enhances the effect of antibiotics, resulting in decreased biofilm biomass and numbers of CFU.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00471-08

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