Affiliation:
1. Department of Microbiology and Cell Science, University of Florida, Kennedy Space Center, Florida 32899
Abstract
ABSTRACT
Bacillus subtilis
is capable of producing 2,3-butanediol from acetoin by fermentation, but to date, the gene encoding the enzyme responsible, acetoin reductase/2,3-butanediol dehydrogenase (AR/BDH), has remained unknown. A search of the
B. subtilis
genome database with the amino acid sequences of functional AR/BDHs from
Saccharomyces cerevisiae
and
Bacillus cereus
resulted in the identification of a highly similar protein encoded by the
B. subtilis ydjL
gene. A knockout strain carrying a
ydjL
::
cat
insertion mutation was constructed, which (i) abolished 2,3-butanediol production in early stationary phase, (ii) produced no detectable AR or BDH activity in vitro, and (iii) accumulated the precursor acetoin in early stationary phase. The
ydjL
::
cat
mutation also affected the kinetics of lactate but not acetate production during stationary-phase cultivation with glucose under oxygen limitation. A very small amount of 2,3-butanediol was detected in very-late-stationary-phase (96-hour) cultures of the
ydjL
::
cat
mutant, suggesting the existence of a second gene encoding a minor AR activity. From the data, it is proposed that the major AR/BDH-encoding gene
ydjL
be renamed
bdhA
.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
144 articles.
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