Rapid diagnosis of enterovirus infection by a new one-step reverse transcription-PCR assay

Author:

Kessler H H1,Santner B1,Rabenau H1,Berger A1,Vince A1,Lewinski C1,Weber B1,Pierer K1,Stuenzner D1,Marth E1,Doerr H W1

Affiliation:

1. Institute of Hygiene, KF-University Graz, Austria. harald.kessler@kfunigraz.ac.at

Abstract

The AMPLICOR Enterovirus Test was evaluated with 103 cerebrospinal fluid (CSF) specimens. Twenty-seven CSF specimens were culture positive. With the AMPLICOR test, enterovirus RNA was detected in 34 specimens. Compared with culture, the AMPLICOR test gave a sensitivity of 96.3% and a specificity of 100%. The sensitivity of culture was 79.4% in comparison with the AMPLICOR test.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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