Determination of anti-streptolysin O antibody titer by a new passive agglutination method using sensitized toraysphere particles

Author:

Kodama T1,Ichiyama S1,Morishita Y1,Fukatsu T1,Shimokata K1,Nakashima N1

Affiliation:

1. Department of Clinical Laboratory Medicine, Nagoya University Hospital, Japan.

Abstract

We developed a new method for determining the anti-streptolysin O (ASO) titer on the basis of a passive agglutination reaction. This new method uses Toraysphere particles sensitized with streptolysin O. This method was used to measure the ASO titer in 130 serum samples (from 31 patients with post-streptococcal glomerulonephritis, 26 patients with group A streptococcal pharyngitis, 5 patients with scarlet fever, 29 patients with non-group A streptococcal pharyngitis, 20 healthy children, and 19 healthy adults). The results were compared with those obtained simultaneously by two conventional methods, the Rantz-Randall and the NA-Latex-ASL methods. The results obtained by the Toraysphere method showed an excellent correlation with those obtained by each conventional method (correlation coefficients, 0.932 for the Rantz-Randall method and 0.913 for the NA-Latex-ASL method). The performance of each method was evaluated with respect to the clinical and microbiological diagnoses for each patient. The sensitivity, specificity, positive predictive value, and negative predictive value for the Toraysphere method were 76, 84, 81, and 79%, respectively. The corresponding values for the Rantz-Randall method were 48, 78, 67, and 62%, respectively, while those for the NA-Latex-ASL method were 60, 82, 76, and 69%, respectively. Because the Toraysphere method is simple and reproducible, has a short reaction time, and has a clear-cut endpoint for the analysis, we recommend it for routine use in determining the ASO titer in the clinical microbiology laboratory.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference15 articles.

Cited by 4 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3