Novel Immunoblot Assay Using Four Recombinant Antigens for Diagnosis of Epstein-Barr Virus Primary Infection and Reactivation-Reference-Cited by-同舟云学术

Novel Immunoblot Assay Using Four Recombinant Antigens for Diagnosis of Epstein-Barr Virus Primary Infection and Reactivation

Published:1999-08 Issue:8 Volume:37 Page:2709-2714
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Buisson M.¹,Fleurent B.²,Mak M.³,Morand P.¹,Chan L.⁴,Ng A.³,Guan M.³,Chin D.³,Seigneurin J. M.¹

Affiliation:

1. Laboratoire de Virologie Médicale Moléculaire, RHAP-CNRS, Faculté de Médecine, 38043 Grenoble, France1;

2. Genelabs Diagnostics S.A., Geneva, Switzerland2; and

3. Genelabs Diagnostics Pte. Ltd.,3 and

4. Bioprocessing Technology Center, National University of Singapore,4 Singapore, Republic of Singapore

Abstract

ABSTRACT A new immunoblot assay, composed of four Epstein-Barr virus (EBV)-encoded recombinant proteins (virus capsid antigen [VCA] p23, early antigen [EA] p138, EA p54, and EBNA-1 p72), was compared with an immunofluorescence assay on a total of 291 sera. The test was accurate in 94.5% of cases of primary EBV infection, while an immunoglobulin G anti-VCA p23 band with strong intensity correlated with reactivation.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.8.2709-2714.1999

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