Engineering of Cyclodextrin Glycosyltransferase through a Size/Polarity Guided Triple-Code Strategy with Enhanced α-Glycosyl Hesperidin Synthesis Ability-Reference-Cited by-同舟云学术

Engineering of Cyclodextrin Glycosyltransferase through a Size/Polarity Guided Triple-Code Strategy with Enhanced α-Glycosyl Hesperidin Synthesis Ability

Published:2022-09-13 Issue:17 Volume:88 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Chen Hanchi¹²^ORCID,Liu Yi¹²,Ren Xiangyi¹²,Wang Jiajun¹²,Zhu Linjiang¹²,Lu Yuele¹²,Chen Xiaolong¹²

Affiliation:

1. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Zhejiang, China

2. Institute of Fermentation Technology, Zhejiang University of Technology, Zhejiang, China

Abstract

Size/polarity guided triple-code strategy, a novel semirational mutagenesis strategy, was developed in this study and employed to engineer the aglycon attacking site of CGTase. Screening pressure was set as improved hesperidin glucoside synthesis ability, and eight positive mutants were obtained by screening only 32 mutants.

Funder

National Natural Science Foundation of China

MOST | 高端外国专家引进计划 | National Ten Thousand Talent Program

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.01027-22

Reference68 articles.

1. The carbohydrate-active enzymes database (CAZy) in 2013

2. α-Amylase: an enzyme specificity found in various families of glycoside hydrolases