Application of PCR to Distinguish Common Species of Dermatophytes-Reference-Cited by-同舟云学术

Application of PCR to Distinguish Common Species of Dermatophytes

Published:2001-09 Issue:9 Volume:39 Page:3382-3385
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Faggi Elisabetta¹,Pini Gabriella¹,Campisi Enza¹,Bertellini Chiara¹,Difonzo Elisa²,Mancianti Francesca³

Affiliation:

1. Dipartimento di SanitàPubblica—Sezione Microbiologia, Università di Firenze, 50134 Florence,1

2. Dipartimento di Scienze Dermatologiche, Università di Firenze, 50121 Florence,2 and

3. Dipartimento di Patologia Animale, Università di Pisa, 56124 Pisa,3 Italy

Abstract

ABSTRACT This report describes the application of PCR fingerprinting for the identification of species and varieties of common dermatophytes and related fungi utilizing as a single primer the simple repetitive oligonucleotide (GACA) 4 . The primer was able to amplify all the strains, producing species-specific profiles for Microsporum canis , Microsporum gypseum , Trichophyton rubrum , Trichophyton ajelloi , and Epidermophyton floccosum. Intraspecific variability was not observed for these species. Instead, three different profiles were observed in the Trichophyton mentagrophytes group.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.9.3382-3385.2001

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