Colorimetric Assay for Antifungal Susceptibility Testing of Aspergillus Species-Reference-Cited by-同舟云学术

Colorimetric Assay for Antifungal Susceptibility Testing of Aspergillus Species

Published:2001-09 Issue:9 Volume:39 Page:3402-3408
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Meletiadis Joseph¹,Mouton Johan W.²,Meis Jacques F. G. M.³,Bouman Bianca A.¹,Donnelly J. Peter³,Verweij Paul E.¹,

Affiliation:

1. Departments of Medical Microbiology1 and

2. Department of Medical Microbiology and Regional Public Health Laboratory, Canisius Wilhelmina Hospital,2 Nijmegen, The Netherlands

3. Hematology,3 University Medical Center Nijmegen, and

Abstract

ABSTRACT A colorimetric assay for antifungal susceptibility testing of Aspergillus species ( Aspergillus fumigatus , Aspergillus flavus , Aspergillus terreus , Aspergillus nidulans , and Aspergillus ustus ) is described based on the reduction of the tetrazolium salt 2,3-bis(2-methoxy-4-nitro-5-[(sulphenylamino)carbonyl]-2H-tetrazolium-hydroxide (XTT) in the presence of menadione as an electron-coupling agent. The combination of 200 μg of XTT/ml with 25 μM menadione resulted in a high production of formazan within 2 h of exposure, allowing the detection of hyphae formed by low inocula of 10 2 CFU/ml after 24 h of incubation. Under these settings, the formazan production correlated linearly with the fungal biomass and less-variable concentration effect curves for amphotericin B and itraconazole were obtained.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.9.3402-3408.2001

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