SILAC analysis of <i>Escherichia coli</i> proteome during progression of growth from exponential to prolonged stationary phase-Reference-Cited by-同舟云学术

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SILAC analysis of Escherichia coli proteome during progression of growth from exponential to prolonged stationary phase

Published:2024-06-11 Issue:6 Volume:13 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Reier Kaspar¹^ORCID,Liiv Aivar¹,Remme Jaanus¹^ORCID

Affiliation:

1. Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia

Abstract

ABSTRACT The expression level of individual proteins varies markedly during the progression of the growth phase in bacteria. A set of proteins was quantified in Escherichia coli total proteome during 14 days of batch cultivation using pulse stable isotope labeled amino acids in cell culture (SILAC)-based quantitative mass spectrometry.

Funder

Eesti Teadusagentuur

Publisher

American Society for Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mra.00042-24

Reference6 articles.

1. The quantitative and condition-dependent Escherichia coli proteome

2. A Conundrum of r-Protein Stability: Unbalanced Stoichiometry of r-Proteins during Stationary Phase in Escherichia coli

3. Bacterial ribosome heterogeneity: Changes in ribosomal protein composition during transition into stationary growth phase

4. Culture Medium for Enterobacteria

5. Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips

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