Affiliation:
1. Yale Arbovirus Research Unit, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06510
Abstract
Chemical and physical variables influencing the plaquing of all dengue serotypes in two simian cell systems were studied. Calf serum in the nutrient overlay may be replaced by mouse ascitic fluid or bovine plasma albumin when employing the rhesus monkey kidney LLC-MK
2
cell system for plaquing all dengue serotypes. Doubling the serum concentration in the overlay had little effect in modifying dengue types 1, 2, 3, and 4 plaque titers. Newborn agamma, 4-week-old and 8-week-old calf serum gave comparable titers with all dengue virus serotypes. Dengue virus titers, plaque size, and development time were unaffected by sodium bicarbonate concentrations ranging from 1.1 to 4.4 mg/ml of overlay. A twofold increase (0.00332 g%) in the amount of either autoclaved or filtered-sterilized neutral red reduced the dengue 2 virus titer as much as 2.2 logs. An increased Mg
++
and decreased Ca
++
concentration in the overlay medium increased the efficiency of the plaquing system.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference19 articles.
1. Plaque assay procedure for Colorado tick fever virus;Dieg E. F.;J. Bacteriol.,1964
2. Nutrition needs of mammalian cells in tissue culture;Eagle H.;Science,1955
3. Dengue virus plaque formation in rhesus monkey kidney cultures;Georgiades J.;Proc. Soc. Exp. Biol. Med.,1965
4. The adaptation and maintenance of mammalian cells to continuous growth in tissue culture;Hull R. N.;Anat. Rec.,1956
5. Development of plaque techniques, for titration and neutralization test with African horse sickness virus;Hopkins I. G.;Amer. J. Vet. Res.,1966
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献