Effect of Chloramphenicol on Host-Bacteriophage Relationships in the Lactic Streptococci

Author:

Erskine J. M.1

Affiliation:

1. New Zealand Dairy Research Institute, Palmerston North, New Zealand

Abstract

Chloramphenicol (CM)-resistant mutants of Streptococcus lactis strain ML3 were obtained either as a consequence of continuous transfer of the bacteria in broth containing increasing amounts of CM or by selecting for high-level resistant derivatives after mutagenic treatment of the bacteria. Some CM-resistant cells obtained by the first method were also resistant to the homologous bacteriophage. Cells trained to grow in the presence of CM developed resistance to some heterologous attacking phages but not to phage ml 3 . Mutants selected for phage resistance were not resistant to CM. There appear to be two different loci for CM resistance on the bacterial chromosome: the one for high-level resistance is associated with the phage-resistance locus and the other is independent of it. A concentration of CM (280 μg/ml) that was bacteriostatic for ML3 inhibited the intracellular growth of ml 3 phage in strain ML3-CM r I, which had been trained to grow in the presence of that CM concentration, despite the fact that cells of this strain were not phage-resistant per se. The drug had no direct virucidal action and did not prevent adsorption to or penetration of phage into the bacterium. Lysogenization did not occur. It is concluded that the block in phage development probably involves inhibition of synthesis of phage components, either involving deoxyribonucleic acid at an early stage or the phage coat protein at a later one.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference17 articles.

1. Adams M. H. 1959. Bacteriophages. Interscience Publishers New York.

2. Industrial utilization of lactic cultures;Babel F. J.;J. Dairy Sci.,1962

3. Chloramphenicol;Brock T. D.;Bacteriol. Rev.,1961

4. Effect of chloramphenicol on Iysogenization by temperate phage P1;Christensen J. R.;Virology,1957

5. Changes in the bacteriophage sensitivity of lactic streptococci;Collins E. B.;J. Dairy Sci.,1958

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