Interaction of Ceftobiprole with the Low-Affinity PBP 5 of Enterococcus faecium-Reference-Cited by-同舟云学术

Interaction of Ceftobiprole with the Low-Affinity PBP 5 of Enterococcus faecium

Published:2010-02 Issue:2 Volume:54 Page:953-955
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Henry Xavier¹,Amoroso Ana¹,Coyette Jacques¹,Joris Bernard¹

Affiliation:

1. Centre d'Ingénierie des Protéines, Institut de Chimie B6a, Université de Liège, Sart Tilman, B 4000 Liège, Belgium

Abstract

ABSTRACT Ceftobiprole is a new cephalosporin that exhibits a high level of affinity for methicillin-resistant Staphylococcus aureus PBP 2a. It was reported that ceftobiprole did not interact with a mutated form of the low-affinity protein Enterococcus faecium PBP 5 (PBP 5fm) that, when overexpressed, confers a β-lactam resistance phenotype to the bacterium. Our results show that ceftobiprole binds to unmutated PBP 5fm to form a stable acyl-enzyme and that ceftobiprole is able to efficiently kill a penicillin-resistant Enterococcus faecium strain that produces this protein.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00983-09

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