L-histidine utilization in Aspergillus nidulans

Author:

Polkinghorne M A,Hynes M J

Abstract

Histidase activity rather than uptake of L-histidine is the limiting factor for the utilization of histidine as the sole nitrogen source for Aspergillus nidulans. Histidine cannot act as the sole carbon source, and evidence is presented indicating that this is attributable to an inability to convert histidine to L-glutamate in vivo. It has been shown that this fungus lacks an active urocanase enzyme and that histidine is quantitatively converted to urocanate, which accumulates in the extracellular medium. The use of histidine as a nitrogen source is regulated by nitrogen metabolite repression control of histidase synthesis. In addition, evidence for a requirement for a carbon source for histidase synthesis and for a minor form of control by nitrate is presented. The activity of the histidase enzyme is inhibited by micromolar concentrations of the product urocanate and by physiological levels of L-glutamate and L-glutamine.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference57 articles.

1. Analysis of 'acetate non-utilizing (acu) mutants in Asperg'illui.s nidulan.s;Armitt S.;J. Gen. Microbiol.,1976

2. Arst H. N. Jr. and C. R. Bailey. The regulation of carbon metabolism in A.spergilllus iidila(ins. p. 131-146. In J. E. Smith and J. A. Pateman (ed.) Genetics and physiology of Aspergilllus. The British Mycological Society Symposium series 1. Academic Press Inc. London.

3. Nitrogen metabolite repression in Aspergillus tliduiltans;Arst H. N.;Mol. Gen. Genet.,1973

4. A mutant of Aspergillus niidi/uans lacking NADP-linked glutamate dehydrogenase;Arst H. N.;Mol. Gen. Genet.,1973

5. Possible failure of NADP-glutamate dehydrogenase to participate directly in nitrogen repression of the allantoin degradative enzymes in SaccharomNices cerevisiae;Bossinger J.;Biochem. Biophys. Res. Commun.,1975

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