Impact of Species Diversity on the Design of RNA-Based Diagnostics for Antibiotic Resistance in Neisseria gonorrhoeae-Reference-Cited by-同舟云学术

Impact of Species Diversity on the Design of RNA-Based Diagnostics for Antibiotic Resistance in Neisseria gonorrhoeae

Published:2019-08 Issue:8 Volume:63 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Wadsworth Crista B.¹^ORCID,Sater Mohamad R. A.¹^ORCID,Bhattacharyya Roby P.²³^ORCID,Grad Yonatan H.¹⁴^ORCID

Affiliation:

1. Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA

2. Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA

3. Division of Infectious Diseases and Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA

4. Division of Infectious Diseases, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA

Abstract

Quantitative assessment of antibiotic-responsive RNA transcripts holds promise for a rapid point-of-care (POC) diagnostic tool for antimicrobial susceptibility testing. These assays aim to distinguish susceptible and resistant isolates by transcriptional differences upon drug exposure. However, an often-overlooked dimension of designing these tests is that the genetic diversity within a species may yield differential transcriptional regulation independent of resistance phenotype.

Funder

HHS | National Institutes of Health

Richard and Susan Smith Family Foundation

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00549-19

Reference47 articles.

1. RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities

2. Rapid Phenotypic Antibiotic Susceptibility Testing Through RNA Detection