Immunization of Mice Against Encephalomyocarditis Virus I. Purification, Concentration, and Inactivation of Encephalomyocarditis Virus-Reference-Cited by-同舟云学术

Immunization of Mice Against Encephalomyocarditis Virus I. Purification, Concentration, and Inactivation of Encephalomyocarditis Virus

Published:1972-10 Issue:4 Volume:6 Page:508-512
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Bogaerts W. J. C.¹,Durville-van der Oord B. J.¹

Affiliation:

1. Medical Biological Laboratory TNO, Department of Microbiology, 139, Lange Kleiweg, Rijswijk (ZH), The Netherlands

Abstract

Methods are described for the concentration, purification, and inactivation of encephalomyocarditis virus grown in L-cell suspension cultures. Virus concentrates were obtained by the precipitation of crude virus lysates with polyethylene glycol and were purified by Genetron extraction. After column chromatography approximately 70% recovery of infectivity and hemagglutination (HA) activity was found. Exposure of the purified virus suspensions to ultraviolet light resulted in reduction of the infectivity by a factor of 10 9 with little or no loss of HA activity. The preparations are being evaluated as vaccines.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.6.4.508-512.1972

Reference21 articles.

1. Aleksandrova G. I. A. A. Smorodintsev N. M. Beljaeva B. J. Vasil'ev R. A. Geft V. G. Panteleev M. A. Sejfer and A. A. Selivanov. 1970. Testing the safety and effectiveness of oral administration of a live influenza vaccine. Bull. W. H. 0. 42: 429-436.

2. Discussion. (Aerosol vaccination with tetanus toxoid.);Bartlema H. C.;Bacteriol. Rev.,1966

3. Beare A. S. D. Hobson S. E. Reed and D. A. J. Tyrrell. 1969. Antibody responses to and efficacy of an inactivated spray vaccine. Bull. W. H. 0. 41:549-551.

4. Antibody responses in serum and nasal secretions of children immunized with inactivated and attenuated measles-virus vaccines;Bellanti J. A.;N. Engl. J. Med.,1969

5. Bowen E. J. 1946. The chemical aspects of light. Clarendon Press Oxford.

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