Detection of Borreliacidal Antibodies in Lyme Borreliosis Patient Sera Containing Antimicrobial Agents-Reference-Cited by-同舟云学术

Detection of Borreliacidal Antibodies in Lyme Borreliosis Patient Sera Containing Antimicrobial Agents

Published:1999-11 Issue:6 Volume:6 Page:930-933
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Jobe Dean A.¹,Rawal Nenoo¹,Schell Ronald F.²³,Callister Steven M.¹⁴

Affiliation:

1. Microbiology Research Laboratory1 and

2. Wisconsin State Laboratory of Hygiene2 and

3. Department of Medical Microbiology and Immunology,3University of Wisconsin, Madison, Wisconsin 53706

4. Section of Infectious Diseases,4 Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601, and

Abstract

ABSTRACT The borreliacidal-antibody test has been used for the serological detection and confirmation of Lyme borreliosis. However, the presence of antimicrobial agents in serum can confound the accurate detection of borreliacidal antibodies. In this study, we developed a Bacillus subtilis agar diffusion bioassay to detect small concentrations of antimicrobial agents in serum. We also used XAD-16, a nonionic polymeric resin, to adsorb and remove high concentrations of amoxicillin, cefotaxime, ceftriaxone, cefuroxime, doxycycline, and erythromycin without significantly affecting even small concentrations of immunoglobulin M (IgM) or IgG borreliacidal antibodies. High concentrations of penicillin could also be removed by adding 1 U of penicillinase without significantly influencing the levels of borreliacidal antibodies. These simple procedures greatly enhance the clinical utility of the borreliacidal-antibody test.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.6.6.930-933.1999

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