Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes-Reference-Cited by-同舟云学术

Genome Sequence of Azotobacter vinelandii , an Obligate Aerobe Specialized To Support Diverse Anaerobic Metabolic Processes

Published:2009-07-15 Issue:14 Volume:191 Page:4534-4545
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Setubal João C.¹²,dos Santos Patricia³,Goldman Barry S.⁴,Ertesvåg Helga⁵,Espin Guadelupe⁶,Rubio Luis M.⁷,Valla Svein⁵,Almeida Nalvo F.¹⁸,Balasubramanian Divya⁹,Cromes Lindsey⁹,Curatti Leonardo¹⁰,Du Zijin⁴,Godsy Eric⁴,Goodner Brad⁹,Hellner-Burris Kaitlyn⁹,Hernandez José A.¹¹,Houmiel Katherine¹²,Imperial Juan¹³,Kennedy Christina¹⁴,Larson Timothy J.¹⁵,Latreille Phil⁴,Ligon Lauren S.¹⁵,Lu Jing⁴,Mærk Mali⁵,Miller Nancy M.⁴,Norton Stacie⁴,O'Carroll Ina P.¹⁵,Paulsen Ian¹⁶,Raulfs Estella C.¹⁵,Roemer Rebecca⁹,Rosser James¹²,Segura Daniel⁶,Slater Steve¹⁷,Stricklin Shawn L.⁴,Studholme David J.¹⁸,Sun Jian¹,Viana Carlos J.⁸,Wallin Erik⁹,Wang Baomin¹⁴,Wheeler Cathy⁹,Zhu Huijun⁴,Dean Dennis R.¹⁵,Dixon Ray¹⁹,Wood Derek¹²²⁰

Affiliation:

1. Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

2. Department of Computer Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

3. Department of Chemistry, Wake Forest University, Winston-Salem, North Carolina 27109

4. Monsanto Company, St. Louis, Missouri

5. Department of Biotechnology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway

6. Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico

7. IMDEA Energia, Centro de Biotecnologia y Genomica de Plantas, Madrid 28223, Spain

8. Departmento de Computacao e Estatistica, Universidade Federal do Mato Grosso do Sul, Campo Grande, Brazil

9. Department of Biology, Hiram College, Ohio

10. CONICET-FIBA, Centro de Estudios de Biodiversidad y Biotecnologia, Mar del Plata 7600, Argentina

11. Department of Biochemistry, Midwestern University, Glendale, Arizona 85308

12. Seattle Pacific University, Seattle, Washington 98026

13. Centro de Biotecnologia y Genomica de Plantas (CBGP, UPM-INIA), Madrid 28223, Spain

14. Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721

15. Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

16. Macquarie University, Sydney, Australia

17. Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin

18. The Sainsbury Laboratory, Colney Lane, Norwich NR4 7UH, United Kingdom

19. Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, United Kingdom

20. Department of Microbiology, University of Washington, Seattle, Washington 98119

Abstract

ABSTRACT Azotobacter vinelandii is a soil bacterium related to the Pseudomonas genus that fixes nitrogen under aerobic conditions while simultaneously protecting nitrogenase from oxygen damage. In response to carbon availability, this organism undergoes a simple differentiation process to form cysts that are resistant to drought and other physical and chemical agents. Here we report the complete genome sequence of A. vinelandii DJ, which has a single circular genome of 5,365,318 bp. In order to reconcile an obligate aerobic lifestyle with exquisitely oxygen-sensitive processes, A. vinelandii is specialized in terms of its complement of respiratory proteins. It is able to produce alginate, a polymer that further protects the organism from excess exogenous oxygen, and it has multiple duplications of alginate modification genes, which may alter alginate composition in response to oxygen availability. The genome analysis identified the chromosomal locations of the genes coding for the three known oxygen-sensitive nitrogenases, as well as genes coding for other oxygen-sensitive enzymes, such as carbon monoxide dehydrogenase and formate dehydrogenase. These findings offer new prospects for the wider application of A. vinelandii as a host for the production and characterization of oxygen-sensitive proteins.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00504-09

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