Bacterial Diversity of Water and Sediment Samples from Gull Point State Park (West Okoboji, Iowa) Determined Using 16S rRNA Gene Amplicon Sequencing

Author:

Kyndt John A.1ORCID

Affiliation:

1. College of Science and Technology, Bellevue University, Bellevue, Nebraska, USA

Abstract

Gull Point State Park is located on a peninsula on the west shore of West Okoboji Lake (Iowa, USA). It is the primary state park in the Iowa Great Lakes region. Sediment and water samples from three locations at the Gull Point pond were analyzed for their microbial composition.

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Reference18 articles.

1. Seigley LS Quade DJ . 1996. Gull Point State Park: a glacial legacy. Iowa Department of Natural Resources. https://web.archive.org/web/20101229011454/http://www.igsb.uiowa.edu/Browse/gullpt/gullpt.htm.

2. Iowa Geological Survey. Geology of Iowa State Parks: Gull Point. https://iowageologicalsurvey.org/iowa-state-parks/gull-point-state-park-a-glacial-legacy/.

3. Illumina. 16S metagenomic sequencing library preparation. https://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf.

4. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

5. Ribosomal Database Project: data and tools for high throughput rRNA analysis

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