Isolation and genomic characterization of S taphylococcus aureus bacteriophages from Chennai, India

Author:

Deepak Shankaregowdanakopalu Jagadeesh1ORCID,Kannan Porteen1ORCID,Savariraj Wilfred Ruban2,S Anbazhagan3,Ayyasamy Elango4,Tuticorin Maragatham Alagesan Senthil Kumar5,Ravindran Narendra Babu6,Sundaram Sureshkannan1,Mohanadasse Nithya Quintoil7,Shippy Teresa D.8,Cull Charley A.9,Levent Gizem10,Amachawadi Raghavendra G.11ORCID

Affiliation:

1. Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, TANUVAS, Chennai, India

2. Department of Livestock Products and Technology, Veterinary College, KVAFSU, Bengaluru, India

3. ICMR-National Animal Resource Facility for Biomedical Research (NARFBR), Hyderabad, Telangana, India

4. Veterinary College and Research Institute, TANUVAS, Salem, India

5. Zoonoses Research Laboratory, Centre for Animal Health Studies, TANUVAS, Chennai, India

6. Department of Livestock Products and Technology, Madras Veterinary College, TANUVAS, Chennai, India

7. Department of Veterinary Public Health and Epidemiology, RIVER, Pondicherry, India

8. Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, Kansas, USA

9. Midwest Veterinary Services, Inc., Oakland, Nebraska, USA

10. Texas Tech University School of Veterinary Medicine, Amarillo, Texas, USA

11. Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA

Abstract

ABSTRACT We isolated and characterized two lytic bacteriophages against Staphylococcus aureus named TANUVAS_MVC-VPHSA1 and TANUVAS_MVC-VPHSA2, with the aim of investigating their genomic and structural features. The bacteriophages belong to the Caudoviricetes, and their genomes have sizes of 50,505 and 50,516 base pairs with a GC content of 41.4%.

Funder

USDA National Institute of Food and Agriculture

HHS | NIH | OSC | Common Fund

National Science Foundation

Institutional Development Award (IDeA)| National Institute of General Medical Sciences| NIH| Kansas| INBRE

Publisher

American Society for Microbiology

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