Characterization of the Mycobacterial NER System Reveals Novel Functions of the uvrD1 Helicase-Reference-Cited by-同舟云学术

Characterization of the Mycobacterial NER System Reveals Novel Functions of the uvrD1 Helicase

Published:2009-01-15 Issue:2 Volume:191 Page:555-562
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Güthlein Carolin¹,Wanner Roger M.¹,Sander Peter¹²,Davis Elaine O.³,Bosshard Martin¹,Jiricny Josef⁴,Böttger Erik C.¹²,Springer Burkhard¹²

Affiliation:

1. Institut für Medizinische Mikrobiologie, University of Zurich, Gloriastrasse 30/32, CH-8006, Zurich, Switzerland

2. Nationales Zentrum für Mykobakterien, Gloriastrasse 30, CH-8006 Zurich, Switzerland

3. Division of Mycobacterial Research, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom

4. Institute of Molecular Cancer Research, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, and Department of Biology, Swiss Federal Institutes of Technology (ETH), CH-8093 Zurich, Switzerland

Abstract

ABSTRACT In this study, we investigated the role of the nucleotide excision repair (NER) pathway in mycobacterial DNA repair. Mycobacterium smegmatis lacking the NER excinuclease component uvrB or the helicase uvrD1 gene and a double knockout lacking both genes were constructed, and their sensitivities to a series of DNA-damaging agents were analyzed. As anticipated, the mycobacterial NER system was shown to be involved in the processing of bulky DNA adducts and interstrand cross-links. In addition, it could be shown to exert a protective effect against oxidizing and nitrosating agents. Interestingly, inactivation of uvrB and uvrD1 significantly increased marker integration frequencies in gene conversion assays. This implies that in mycobacteria (which lack the postreplicative mismatch repair system) NER, and particularly the UvrD1 helicase, is involved in the processing of a subset of recombination-associated mismatches.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00216-08

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