In Vitro Synthesis of Turnip Yellow Mosaic Virus Coat Protein in a Wheat Germ Cell-Free System-Reference-Cited by-同舟云学术

In Vitro Synthesis of Turnip Yellow Mosaic Virus Coat Protein in a Wheat Germ Cell-Free System

Published:1976-10 Issue:1 Volume:20 Page:196-202
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Benicourt C.¹,Haenni A. L.¹

Affiliation:

1. Laboratoire de Biochimie du Développement, Institut de Biologie Moléculaire du Centre National de la Recherche Scientifique, Université Paris VII, 75005 Paris, France

Abstract

Turnip Yellow Mosaic Virus RNA directs the synthesis in vitro of its coat protein in a wheat germ cell-free extract. Optimum conditions for synthesis have been defined, and the effect of spermine on specifically enhancing coat protein formation has been examined. Identity between the in vitro synthesized coat protein and authentic coat protein of Turnip Yellow Mosaic Virus was established by analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peptide mapping, and immunoprecipitation.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.20.1.196-202.1976

Reference33 articles.

1. Benicourt C. and A. L. Haenni. 1975. Translation of TYMV RNA in a wheat germ cell-free system p. 189-196. In A. L. Haenni and G. Beaud (ed.) In vitro transcription and translation of viral genomes vol. 47. INSERM Paris.

2. Blackburn S. 1965. The determination of amino acids by high-voltage paper electrophoresis p. 1-45. In D. Glick (ed.) Methods of biochemical analysis vol. 13. Interscience Publishers New York.

3. Bacteriophage Q,8 replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts;Blumenthal T.;Proc. Natl. Acad. Sci. U.S.A.,1972

4. Synthesis of polyoma protein in vitro;Crawford L. V.;J. Mol. Biol.,1973

5. Translation of virus mRNA: synthesis of bacteriophage Q/3 proteins in a cell-free extract from wheat embryo;Davies J. W.;J. Virol.,1973

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