Cre Reporter System To Monitor the Translocation of Type III Secreted Proteins into Host Cells-Reference-Cited by-同舟云学术

Cre Reporter System To Monitor the Translocation of Type III Secreted Proteins into Host Cells

Published:2006-02 Issue:2 Volume:74 Page:1084-1090
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Briones Gabriel¹,Hofreuter Dirk¹,Galán Jorge E.¹

Affiliation:

1. Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven, Connecticut 06536

Abstract

ABSTRACT Central to the study of type III secretion systems is the availability of reporter systems to monitor bacterial protein translocation into host cells. We report here the development of a bacteriophage P1 Cre recombinase-based system to monitor the translocation of bacterial proteins into mammalian cells. Bacteriophage P1 Cre recombinase fused to the secretion and translocation signals of Salmonella enterica serovar Typhimurium of the type III secreted protein SopE was secreted in a type III secretion system-dependent fashion. More importantly, the SopE-Cre chimera was translocated into host cells via the type III secretion system and activated the expression of luciferase and green fluorescent protein reporters of Cre recombinase activity.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.74.2.1084-1090.2006

Reference33 articles.

1. Identification of the Secretion and Translocation Domain of the Enteropathogenic and Enterohemorrhagic Escherichia coli Effector Cif, Using TEM-1 β-Lactamase as a New Fluorescence-Based Reporter

2. Chen, L. M., K. Kaniga, and J. E. Galán. 1996. Salmonella spp. are cytotoxic for cultured macrophages. Mol. Microbiol.21:1101-1115.

3. Cornelis, G. R., and F. Van Gijsegem. 2000. Assembly and function of type III secretory systems. Annu. Rev. Microbiol.54:735-774.

4. Day, J. B., F. Ferracci, and G. V. Plano. 2003. Translocation of YopE and YopN into eukaryotic cells by Yersinia pestis yopN, tyeA, sycN, yscB and lcrG deletion mutants measured using a phosphorylatable peptide tag and phosphospecific antibodies. Mol. Microbiol.47:807-823.

5. Mucosal Priming of Simian Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Responses in Rhesus Macaques by the Salmonella Type III Secretion Antigen Delivery System

Cited by 13 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Recent Advancements in Tracking Bacterial Effector Protein Translocation;Microorganisms;2022-01-24

2. Imaging of anthrax intoxication in mice reveals shared and individual functions of surface receptors CMG-2 and TEM-8 in cellular toxin entry;Journal of Biological Chemistry;2022-01

3. Direct imaging of anthrax intoxication in animals reveals shared and individual functions of CMG-2 and TEM-8 in cellular toxin entry;2021-01-22

4. Bacterial adhesion and host cell factors leading to effector protein injection by type III secretion system;International Journal of Medical Microbiology;2019-07

5. Tracking elusive cargo: Illuminating spatio-temporal Type 3 effector protein dynamics using reporters;Cellular Microbiology;2017-11-23