Structural Basis for Selective Oxidation of Phosphorylated Ethylphenols by Cytochrome P450 Monooxygenase CreJ

Author:

Dong Sheng12,Chen Jingfei12,Zhang Xingwang3,Guo Fei3,Ma Li3,You Cai3,Wang Xiao4,Zhang Wei35,Wan Xiaobo16,Liu Shuang-Jiang72ORCID,Yao Li-Shan12,Li Shengying38,Du Lei139,Feng Yingang12ORCID

Affiliation:

1. Shandong Provincial Key Laboratory of Synthetic Biology, CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China

2. University of Chinese Academy of Sciences, Beijing, China

3. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, China

4. Qingdao Industrial Energy Storage Research Institute, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China

5. The State Key Laboratory of Bio-organic and Natural Products Chemistry, Chinese Academy of Sciences, Shanghai, China

6. Laboratory of Optoelectronic Chemical Materials and Devices, Ministry of Education, School of Chemical & Environmental Engineering, Jianghan University, Wuhan, China

7. State Key Laboratory of Microbial Resources, and Environmental Microbiology and Biotechnology Research Center at Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

8. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong, China

9. Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China

Abstract

The key cytochrome P450 monooxygenase CreJ showed excellent regio- and stereoselectivity in the oxidation of various alkylphenol substrates. C-H bond functionalization of these toxic alkylphenols holds great significance both for biological degradation of these environmental chemicals and production of value-added structural derivatives in pharmaceutical and biochemical industries.

Funder

Shandong Provincial Natural Science Foundatuion

QIBEBT and Dalian National Laboratory For Clean Energy

State Key Laboratory of Bio-organic and Natural Products Chemistry

Director Innovationa Foundation of Qingdao Institute of Bioenergy and Bioprocess Technology

China Postdoctoral Science Foundation | National Postdoctoral Program for Innovative Talents

MOST | National Key Research and Development Program of China

National Natural Science Foundation of China

SDU | Fundamental Research Fund of Shandong University

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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